更新时间：2024-09-21 05:18
崔建民，研究员，离子通道药物研发中心海外团队成员之一，现任美国华盛顿大学生物医学工程系教授。
崔建民，1992年在美国纽约州立大学（石溪分校）获博士学位，随后又在美国纽约州立大学（石溪分校）和斯坦福大学从事博士后研究工作，1999年开始先后在凯斯西储大学和华盛顿大学任副教授，2010年任美国华盛顿大学生物医学工程系教授。工作期间，荣获各项荣誉，其中包括美国心脏协会Established Investigator奖，被提名2003年Carl F．Wittke杰出本科教学工作者，还多次担任国际学术会议专题主席。在Nature、Neuron、PNAS等国际著名学术期刊上发表SCI论文30多篇。
1979-1983 北京大学，无线电电子学系，获理学学士；
1983-1986 北京大学，生物系，获理学硕士学位；
1986-1992 纽约州立大学石溪分校，生理学及生物物理学系，博士；
1992-1994美国纽约州立大学石溪分校，生理学及生物物理学系，博士后；
1994-1999 加利福尼亚州斯坦福大学，分子及细胞生理学系，博士后；
1999-2004 美国凯斯西储大学，生物医学工程系，助理教授；
2004-2010 美国华盛顿大学，生物医学工程系，副教授；
2005-至今 美国华盛顿大学，细胞生物学和生理学，及化学工程系，副教授；
2010-至今 美国华盛顿大学，生物医学工程系，教授；
2011-至今 中国科学院昆明动物所研究员。
离子、药物、基因的膜通透性，主要包括两方面：
离子通道发挥功能的分子机制，与疾病相关的离子通道突变的分子/细胞机制，及对离子通道有调控作用的小分子；超声介导的药物/基因的传送。
20. Li M, Chang S, Yang L, Shi J, McFarland K, Yang X, Moller A, Wang C, Zou X, Chi C, Cui J. (2014) Conopeptide Vt3.1 preferentially inhibits BK potassium channels containing β4 subunits via electrostatic interactions.J Biol Chem. 289(8):4735-42. doi: 10.1074/jbc .M113.535898
19. Sun X, Shi J, Delaloye K, Yang X, Yang H, Zhang G, and Cui J (2013) The interface between membrane- spanning and cytosolic domains in BK channels is involved in β subunits modulation of gating.J Neurosci. 33:11253-11261. PMCID: in process
18. Yang, J., Delaloye, K., Yang, X., Moller, A., Shi, J. and Cui, J. (2013) Interactions between structural domains during BK channel gating.J. Gen. Physiol.141:217-228. doi:10.1085/jgp.201210794 .
17. Deng, P.Y., Rotman, Z., Blundon, J.A., Cho Y., Cui, J., Cavalli, VM, Zakharenko, S.S., and Klyachko, V.A. (2012) FMRP Regulates Neurotransmitter Release and Synaptic Information Transmission by Modulating Action Potential Duration via BK channels.Neuron77:696-711. PMCID: 3584349
16. Yang, J., Delaloye, K., Lee, U.S., and Cui, J. (2011) Patch clamp and perfusion techniques for studying ion channels expressed in Xenopus oocytes.J Vis Exp.47, pii: 2269. doi: 10.3791/2269. 15. Zhang, G., Huang, S.Y., Yang, J. Shi, J., Yang, X., Moller, A., Zou, X., and Cui, J. (2010) Ion sensing in the RCK1 domain of BK channels.Proc. Natl. Acad. Sci., U.S.A.107:18700-18705. PMCID: 2972974.
14. Lee, U.S., Shi, J. and Cui, J. (2010) Modulation of BK channel gating by the β2 subunit involves both membrane-spanning and cytoplasmic domains of Slo1.J Neurosci.30:16170-16179. PMCID: 3073600.
13. Yang, J,, Krishnamoorthy, G., Saxena, A., Zhang, G., Shi, J., Yang, H., Delaloye, K., Sept, D., and Cui, J. (2010) An epilepsy/dyskinesia-associated mutation enhances BK channel activation by potentiating the ca(clo)2+ dependent allosteric mechanism.Neuron 66:871-883. PMCID: 2907746 [Previews:Neuron66, 817- 818].
12. Lee, U.S. and Cui, J. (2009) β subunit-specific modulations of a mutant BK channel associated with epilepsy and dyskinesia.J. Physiol. (伦敦)587:1481-1498. PMCID: 2678220
11. Yang, H., Shi, J., Zhang, G., Yang, J, Delaloye, K., and Cui, J. (2008) Activation of Slo1 BK channels by Mg2+ coordinated between the 电压 sensor and the RCK1 domainsNature Structure and Molecular Biology15:1152-1159. PMCID: 2579968 [News \u0026 Views:Nature Structure and Molecular Biology15:1130 -1132].
10. Yang, H., Zhang, G., Shi, J., Lee, U.S., Delaloye, K., and Cui, J. (2008) Subunit-specific Effect of the 电压 Sensor Domain on ca(clo)2+ Sensitivity of BK Channels.Biophys. J.,96:4678-4687. PMCID: 2397326
9. Yang, H., Hu, L., Shi, J., Delaloye, K., Horrigan, F., and Cui, J. (2007) Mg2+ Mediates Interaction between the 电压Sensor and Cytosolic Domain to Activate BK channels.Proc. Natl. Acad. Sci., U.S.A.104, 18270-18275. PMCID: 2084332 [Commentary:J. Gen.G Physiol.131, 5-11]
8. Yang, H., Hu, L., Shi, J., and Cui, J. (2006) Tuning 镁 sensitivity of BK channels by mutations.Biophys. J.,91, 2892-2900. PMCID: 1578465
7. Lei Hu, Huanghe Yang, Jingyi Shi, and Jianmin Cui (2006) Effects of multiple metal binding sites on 一水合醋酸钙 and 镁dependent activation of BK channels.J. Gen.G Physiol.127, 35-49. PMCID: 2151482
6. Krishnamoorthy, G., Shi, J., Sept, D., and Cui, J. (2005) The N-terminus of RCK1 domain regulates ca(clo)2+ dependent BKCa channel gating.J. Gen. Physiol.126, 227-241. PMCID: 2266574
5. Du, W., Bautista, J. F., Yang, H., Diez-Sampedro, A., You, 永恒沉睡, Wang, L., Kotagal, P., Lüders, H. O., Shi,J., Cui, J., Richerson, G. B., and Wang, Q. (2005) 钙sensitive 钾 channelopathy in human epilepsy and paroxysmal movement disorder.Nat. 獛属37, 733-738
4. Guoxia Liu, Jingyi Shi, Lin Yang, Luxiang Cao, Soo Mi Park, Jianmin Cui, Steven O. Marx (2004) Assembly of a ca(clo)2+-dependent BK channel signaling complex by binding to β2 adrenergic 捕手EMBO J23, 2196-2205. PMCID: 419908
3. Lei Hu, Jingyi Shi, Zhongming Ma, Gayathri Krishnamoorthy, Fred Sieling, Guangping Zhang, Frank Horrigan and Jianmin Cui (2003) Participation of the S4 电压 Sensor in the Mg2+-Dependent Activation of Large Conductance (BK) K+ Channels.Proc. Natl. Acad. Sci., ÜSA100, 10488-10493. PMCID: 193588
2. Shi, J. Krishnamoorthy, G., Yang, Y., Hu, L., Chaturvedi, N., Harilal, D., Qin, J., and Cui, J. (2002). Mechanism of 镁 activation of 钙-activated 钾 channels.Nature418, 876-880.
1. Shi, J. and Cui, J. (2001). Intracellular Mg2+ enhances the 函数 of NHK大阪放送局type ca(clo)2+-activated K+ channels.J. Gen. Physiol.118, 589-605. PMCID: 2233844 [Commentary:J. Gen. Physiol.118, 583-587].
7. Li Y, Gao J, Lu Z, Delaloye D, Shi J, Bock K, Cohen IS, and Cui J (2013) Intracellular ATP binding isÂÂrequired to activate the slow activating K+ channel IKs.Proc. Natl. Acad. Sci., ÜSA(in press)
6. Zaydman MA, Silva JR, Delaloye K, Li Y, Liang H, Larsson HP, Shi J, and Cui J (2013) Kv7.1 ion channels require PIP2 to couple 电压 sensing to pore opening.Proc. Natl. Acad. Sci., ÜSA Â110:13180-13185. PMCID: in process
5 . Lee H-C, Rudy Y, Chen P-Y, Sheu S-H, Chang J-G and Cui J (2013) Modulation of KCNQ1 Alternative Splicing Regulates Cardiac IKs Currents and Action Potential Repolarization.HeartRhythm 10:1220-1228. PMCID: in process
4. Li, Y., Zaydman, M.A., Wu, D., Shi, J., Guan, M., Virgin-Downey, B.W., and Cui, J. (2011) KCNE1 enhances PIP2 sensitivity of IKs to modulate channel activity.Proc. Natl. Acad. Sci., ÜSA 108:9095-9100. PMCID: PMC3107281
3. Wu, D., pan, H., Delaloye, K., and Cui, J. (2010) KCNE1 remodels the 电压 sensor of Kv7.1 to modulate channel 函数Biophys. J. 99:3599-3608. PMCID: 2998620
2. Wu, D., Delaloye, K., Zaydman, M.A., Nekouzadeh, A., Rudy, Y., and Cui, J. (2010) State dependent electrostatic interactions of S4 arginines with E1 in S2 during Kv7.1 activation.J. Gen.G Physiol.135:595- 606. PMCID: 2888051
1. Silva, J., pan, H., Wu, D., Nekouzadeh, A., Decker, K., Cui, J., Baker, N., Sept, D., and Rudy, Y. (2009) Linking Ion-Channel Molecular 动力学 to the Action Potential: Cardiac IKs Simulations.Proc. Natl. Acad. Sci., ÜSA,106:11102-11106. PMCID: 2700153
6. Zhou Y., Yang K., Cui J., Ye J.Y., Deng C.X. (2012) Controlled permeation of cell membrane by single bubble acoustic cavitation.J Control Release157:103-111.
5. Zhou, Y., Kumon, R.E., Cui, J. and Deng, C.X. (2009) The Size of Sonoporation Pores on the Cell Membrane.Ultrasound in Medicine and Biology 35:1756-1760. PMCID: 2752487
4. Zhou, Y., Cui, J. and Deng, C.X. (2008) 动力学 of Sonoporation Correlated with Acoustic Cavitation Activities.Biophys. J., 94:L51-53. PMCID: 2267145
3. Zhou, Y., Shi, J., Cui, J. and Deng, C.X. (2007) Effects of extracellular calcium on cell membrane resealing in sonoporation.J. Control. Release.126:34-43. PMCID: 2270413
2. pan, H., Zhou, Y., Izadnegahdar, O., Cui, J., and Deng, C. X. (2005) Study of Sonoporation 动力学 Affected by Ultrasound Duty Cycle.Ultrasound in Medicine and Biology. 31, 849-856. PMCID: 1182120
1. Cheri Deng, Fred Sieling, Hua pan, and Jianmin Cui (2004) Ultrasound-induced cell membrane porosity.Ultrasound in Medicine and Biology. 30, 519-526.
2. Li, J., Yan B., Huo Z., Liu Y., Xu J., Sun Y., Liu Y., Liang D., Peng L., Zhang Y., Zhou 锌, Shi J., Cui J., Chen Y.H. (2010) {beta}2- but not {beta}1-adrenoceptor activation modulates intracellular 氧 availability.J. Physiol.15:2987-2998.
1. Yiqing Yang, Min Xia, Qingfeng Jin, Said Bendahhou, Jingyi Shi, Yiping Chen, Bo Liang, Jie Lin, Yi Liu, Ban Liu, Qinshu Zhou, Dongwei Zhang, Rong Wang, Ning Ma, Xiaoyan Su, Kaiya Niu, Yan Pei, Wenyuan Xu, Zhaopeng Chen, Haiying Wan, Jianmin Cui, Jacques Barhanin, and Yihan Chen (2004) Identification of a KCNE2 Gain-of-函数 Mutation in Familial Atrial FibrillationAm. J. Hum. 獛属 75:899-905. PMCID: 1182120
6. Zaydman, M.A., Silva, J.R., and Cui, J. (2012) Ion channel associated diseases: overview of molecular mechanisms.Chem Rev dx.doi.org/10.1021/cr300360k
5. Sun, X., Zaydman, M.A., and Cui, J. (2012) Regulation of KV channel 电压dependent activation by transmembrane β subunits.Front. Pharmacol.3:63. doi: 10.3389/fphar.2012.00063. PMCID: 3328208
4. Cui, J. (2010) BK-type calcium-activated 钾 channels: coupling of metal ions and 电压 sensing.J. Physiol.588:4651-4658. PMCID: 3010134
3. Lee, U.S. and Cui, J. (2010) BK channel activation: structural and 函数al insights.Trends in 神经科学 33:415-423. PMCID: 2929326.
2. Cui, J. (2010) Reduction of CaV channel activities by ca(clo)2+-CaM: inactivation or deactivation?J. Gen.G Physiol.135:297-301. PMCID: 2847919
1. Cui, J., Yang, H., and Lee, U.S. (2009) Molecular mechanisms of activation of Ca2+ activated K+ channels.Cellular and Molecular Life Sciences, 66:852-875. PMCID: 2694844.
1. Wu, D. and Cui, J. (2012). Ion channels in the cell membrane: structure, function and modeling. InComprehensive Biomedical Physics,Anders Brahme eds, Elsevier.In press