郭国骥(浙江大学医学院教授) - 知识百科yizesci.com

郭国骥，男，1983年4月12日出生，现任浙江大学医学院教授，博士生导师。

2014年由浙江大学高层次人才引进计划从哈佛大学医学院引进并全职回国，成为浙江大学最年轻的教授之一。2015年入选中组部“国家青年”，2017年获“国家优秀青年基金”。其科研团队主要利用单细胞分析技术研究干细胞的再生和分化机制。

人物经历

教育经历

工作经历

2010.8-2010.10新加坡基因研究所博士后

2010.11-2014.9哈佛大学医学院 Research Fellow

2014.10-浙江大学医学院教授、博士生导师

2020年，获“霍英东教育基金会第十七届高等院校青年教师奖”。

主要成就

研究方向

干细胞与再生医学

高通量单细胞分析技术

细胞图谱

人工智能

一、建立了高通量单细胞分析技术的若干实验方法以及数据分析手段。

二、绘制世界首个小鼠细胞图谱和首个人类细胞图谱，并建立基于图谱的单细胞比对系统。

三、利用单细胞分析技术系统性的解析了小鼠造血干细胞的再生与分化机制。

四、利用单细胞分析技术解析了小分子组合诱导重编程的机制，并为再生医学的细胞来源问题提供了新的思路。

郭国骥团队自主研发了低成本、高效率、完全国产化的高通量单细胞分析平台，并利用这一平台构建了全球首个小鼠细胞图谱和首个人类细胞图谱

利用单细胞基因表达谱分析的手段来研。究干细胞的分化路径和分化机制，开辟了单细胞系统生物学新领域。建立并优化了高通量单细胞定量PCR的基本实验方法，以及数据分析手段。构建Microwell-seq高通量单细胞分析平台，并绘制全球首个哺乳纲细胞图谱。在胚胎期干细胞系统和成体造血干细胞系统中发现了谱系基因预表达的现象，解析了这一现象的分子机制，并进一步阐明了其对干细胞终端分化的影响。五年内在Cell, Cell Stem Cell, Developmental Cell等著名期刊发表学术论文10余篇，总影响因子180分，总引用率650余次。

2020年3月26日，世界首个人类细胞图谱日前在浙江大学绘制成功。国际顶级期刊《自然》在线刊登了浙江大学医学院郭国骥教授团队的这项最新研究成果。

发表文章

Han X, Chen H, Huang D, Chen H, Fei L, Cheng C, Huang H, Yuan GC,Guo G. Mapping human pluripotent stem cell differentiation pathways using high throughput single-cell 核糖核酸sequencing. Genome Biol. 2018 Apr 5;19(1):47.

Han X, Wang R, Zhou Y, Fei L, Sun H, Lai S, Saadatpour A, Zhou Z, Chen H, Ye F, Huang D, Xu Y, Huang W, Jiang M, Jiang X, Mao J, Chen Y, Lu C, Xie J, Fang Q, Wang Y, Yue R, Li T, Huang H, Orkin SH, Yuan GC, Chen M,Guo G. Mapping the Mouse Cell Atlas by Microwell-Seq.Cell. 2018 Feb 22; 172(5):1091-1107.e17.

Han X, Yu H, Huang D, Xu Y, Saadatpour A, Li X, Wang L, Yu J, Pinello L, Lai S, Jiang M, Tian X, Zhang F, Cen Y, Fujiwara Y, Zhu W, Zhou B, Zhou T, Ouyang H, Wang J, Yuan GC, Duan S, Orkin SH,Guo G. A molecular roadmap for induced multi-lineage trans-differentiation of fibroblasts by chemical combinations. Cell Res. 2017 Mar;27(3):386-401.

Beyaz S, Mana MD, Roper J, Kedrin D, Saadatpour A, Hong SJ, Bauer-Rowe KE, Xifaras ME, Akkad A, Arias E, Pinello L, Katz Y, Shinagare S, Abu-Remaileh M, Mihaylova MM, Lamming DW, Dogum R,Guo G, Bell GW, Selig M, Nielsen GP, Gupta N, Ferrone CR, Deshpande V, Yuan GC, Orkin SH, Sabatini DM, Yilmaz ÖH. High-fat diet enhances stemness and tumorigenicity of intestinal progenitors. Nature. 2016 Mar 3;531(7592):53-8.

Guo G#，Pinello L, Han X, Lai S, Shen L, Lin TW, Zou K, Yuan GC#, Orkin SH#. Serum-based culture conditions provoke gene expression variability in mouse embryonic stem cells as revealed by single cell analysis. Cell Rep. 2016 Jan 19;14(4):956-65. #Co-corresponding authors.

Saadatpour A, Lai S,Guo G#, Yuan GC#. Single-Cell Analysis in Cancer Genomics. Trends 獛属 2015 Oct;31(10):576-86. doi: 10.1016/j.tig.2015.07.003. #Co-corresponding authors.

Saadatpour A*,Guo G*, Orkin SH, Yuan GC. Characterizing heterogeneity in leukemic cells using single-cell gene expression analysis. Genome Biol. Genome Biol. 2014 Dec 3;15(12):525. doi: 10.1186/s13059-014-0525-9. *世界斯诺克中国公开赛first authors.

Graham SL, Wicher KB, Jedrusik A,Guo G, Herath W, Robson P and Zernicka-Goetz M. BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo. Nat Commun. 2014 Dec 16;5:5667. doi: 10.1038/ncomms6667.

Marco E, Karp RL,Guo G, Robson P, Hart AH, Trippa L, Yuan GC. Bifurcation analysis of single-cell gene expression data reveals epigenetic landscape. Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):E5643-50. doi: 10.1073/pnas.1408993111. Epub 2014 Dec 15.

Lundberg P, Takizawa H, Kubovcakova L,Guo G, Hao-Shen H, Dirnhofer S, Orkin SH, Manz MG, Skoda RC. Myeloproliferative neoplasms can be initiated from a single hematopoietic stem cell expressing JAK2-V617F. J Exp Med. 2014 Oct 20;211(11):2213-30. doi: 10.1084/jem.20131371. Epub 2014 Oct 6.

Riddell J, Gazit R, Garrison BS,Guo G, Saadatpour A, Mandal PK, Ebina W, Volchkov P, Yuan GC, Orkin SH, Rossi DJ. Reprogramming committed murine blood cells to induced hematopoietic stem cells with defined factors. Cell. 2014 Apr 24;157(3):549-64. doi: 10.1016/j.cell.2014.04.006.

Das PP, Shao Z, Beyaz S, Apostolou E, Pinello L, De Los Angeles A, O'Brien K, Atsma JM, Fujiwara Y, Nguyen M, Ljuboja D,Guo G, Woo A, Yuan GC, Onder T, Daley G, Hochedlinger K, Kim J, Orkin SH. Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity. 摩尔 Cell. 2014 Jan 9;53(1):32-48. doi: 10.1016/j.molcel.2013.11.011. Epub 2013 Dec 19.

Guo G, Luc S, Marco E, Lin TW, Peng C, Kerenyi MA, Beyaz S, Kim W, Xu J, Das PP, Neff T, Zou K, Yuan GC, Orkin SH. Mapping cellular hierarchy by single-cell analysis of the cell surface repertoire. Cell Stem Cell. 2013 Oct 3;13(4):492-505. doi: 10.1016/j.stem.2013.07.017. Epub 2013 Sep 12.

Kim W, 鸟纲 GH, Neff T,Guo G, Kerenyi MA, Walensky LD, Orkin SH. Targeted disruption of the EZH2-EED complex inhibits EZH2-dependent cancer. Nat Chem Biol. 2013 Oct;9(10):643-50. doi: 10.1038/nchembio.1331. Epub 2013 Aug 25.

Kerenyi MA, Shao Z, Hsu YJ,Guo G, Luc S, O'Brien K, Fujiwara Y, peng C, Nguyen M, Orkin SH. Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation. Elife. 2013 Jun 18;2:e00633. doi: 10.7554/eLife.00633.

Do DV, Ueda J, Messerschmidt DM, Lorthongpanich C, Zhou Y, Feng B,Guo G, Lin PJ, Hossain MZ, Zhang W, Moh A, Wu Q, Robson P, Ng HH, Poellinger L, Knowles BB, Solter D, Fu XY. A genetic and developmental pathway from STAT3 to the OCT4-NANOG circuit is essential for maintenance of ICM lineages in vivo. Genes Dev. 2013 Jun 15;27(12):1378-90. doi: 10.1101/gad.221176.113.

Maclean GA, Menne TF,Guo G, Sanchez DJ, Park IH, Daley GQ, Orkin SH. Altered hematopoiesis in trisomy 21 as revealed through in vitro differentiation of isogenic human pluripotent cells. Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17567-72. doi: 10.1073/pnas.1215468109. Epub 2012 Oct 8.

Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P. Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell. 2010 Apr 20;18(4):675-85. doi:10.1016/j.devcel.2010.02.012.

Ralston A, Cox BJ, Nishioka N, Sasaki H, Chea E, Rugg-Gunn P,Guo G, Robson P, Draper JS, Rossant J. Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2. Development. 2010 Feb;137(3):395-403. doi:10.1242/dev.038828.

Yuan P, Han J,Guo G, Orlov YL, Huss M, Loh YH, Yaw LP, Robson P, Lim B, Ng HH. Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells. Genes Dev. 2009 Nov 1;23(21):2507-20. doi: 10.1101/gad.1831909.

Jedrusik A, Parfitt DE,Guo G, Skamagki M, Grabarek JB, Johnson MH, Robson P, Zernicka-Goetz M. Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell 质量 in the mouse embryo. Genes dev 2008 Oct 1;22(19):2692-706. doi: 10.1101/gad.486108.